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API 4000 triple quad

M-8000 ion trap Qtof

LC/MS


API 4000 triple quadrupole (Applied Biosystems).

The API 4000 triple quadrupole is an ideal instrument for sensitive target compound analysis. The ionization source of this instrument as well as the mass spectrometer are very robust compared to other instrument designs. Therefore, this apparatus is used for quantification of low concentrated molecules. Research projects on this instrument; folate analysis, antibiotics analysis, taxanes analysis.

M-8000 ion trap based system (Merck-Hitatchi).

This system has the potential to operate with three different interfaces to connect the liquid flow to the mass spectrometer, working in a vacuum. Atmospheric pressure chemical ionization (APCI) ionizes analytes in the gas phase with a needle discharge. Electrospray (ESI) on the other hand uses a high voltage on a capillary, the analyte is charged in the liquid phase. Both these interfaces are available from different manufacturers. Sonic spray ionization (SSI) uses a very high (sonic, the speed of sound) gas flow to deliver the analyte to the mass spectrometer. A major advantage over APCI and ESI lies in the fact that high voltages or high temperatures are not necessary, which allows the analysis of thermally labile compounds.

The mass spectrometer is based in the ion trap technology. By applying specific voltages, ions can be selected and retained in the trap. In this way, selected ions can be fragmented and fragments can be monitored.

More information about the M-8000 can be found on the manufacturers website

Developed analytical methodology on the M-8000:

Articles:

Sonic Spray Ionization Technology: Performance Study and Application to a LC/MS Analysis on a Monolithic Silica Column for Heroin Impurity Profiling. Riet Dams, Tom Benijts, Wolfgang Günther, Willy Lambert, and André De Leenheer. Anal. Chem., 74, 3206-3212 (2002).[Journal abstract]

Analysis of estrogenic contaminants in river water using liquid chromatography coupled to ion trap based mass spectrometry. Tom Benijts, Riet Dams, Wolfgang Günther, Willy Lambert, André De Leenheer. Rapid Commun. Mass Spectrom., 16, 1358 (2002) [Journal abstract]

Influence of the eluent composition on the ionization efficiency for morphine of pneumatically assisted electrospray, atmospheric-pressure chemical ionization and sonic spray. R. Dams 1, T. Benijts 1, W. Günther 2, W. Lambert 1, A. De Leenheer.  Rapid Commun. Mass. Spectrom., 16, 1072 (2002). [Journal abstract]

Determination of paramethoxyamphetamine and other amphetamine-related designer drugs by liquid chromatography/sonic spray ionization mass spectrometry. K.A. Mortier, R. Dams, W.E. Lambert, E.A. De Letter, S. Van Calenbergh, A.P. De Leenheer.  Rapid Commun. Mass. Spectrom., 16, 865 (2002). [Journal abstract]

Oral presentations:

The role of liquid chromatography mass spectrometry in toxicology. Mortier K., Benijts T., Dams R., Lambert W., Presentation for the ‘Nederlandse vereniging van klinisch farmaceutische analisten in Ziekenhuizen’, Enschede, The Netherlands, 09.07.2002 (as invited speaker) [Download presentation]

K.A. Mortier, R. Dams, W.E. Lambert and A.P. De Leenheer. A validated method for the determination of PMA and other amphetamine-related designer drugs in biological matrices by liquid chromatography sonic spray ionization mass spectrometry. Abstracts of the second joint meeting of the BLT and LTG, 08.03.02 - 09.03.02. [Downoad abstract] [Download presentation]

T. Benijts, R. Dams, W. Lambert and A.P. De Leenheer. Applicability of high performance liquid chromatography coupled to ion trap based mass spectrometry in the analysis of endocrine disrupting chemicals. Abstracts book of the Seventh International Symposium on Hyphenated Techniques in Chromatography and hyphenated Chromatographic Analyzers (HTC-7), Brugge (Belgium), 06.02.02 - 08.02.02, (Abstract 18.2, orale presentatie) [Downoad abstract] [Download presentation]

R. Dams, T. Benijts, W.E. Lambert and A.P. De Leenheer.  Simultaneous determination of in total seventeen opium alkaloids and opioids in blood and urine by fast HPLC-DAD-FL detection, after solid-phase extraction.  Abstracts of the 39th TIAFT Meeting, Praag (Tsjechië), 26.08.01 – 30.08.01, p. 60 [Downoad abstract] [Download presentation].

R. Dams, T. Benijts, W.E. Lambert and A.P. De Leenheer.  Performance of the API ion sources, ESI, APCI, and SSI under different eluent conditions for opiate analysis.  Abstracts of the 11th Nordic Conference on Mass Spectrometry, Loen (Noorwegen), 18.08.01-21.08.01, p.27 [Downoad abstract] [Download presentation].

T. Benijts, R. Dams, W.E. Lambert and A.P. De Leenheer.  Liquid chromatography coupled to ion trap based mass spectrometry applied in the analysis of estrogenic contaminants.  Abstracts of the 11th Nordic Conference on Mass Spectrometry, Loen (Noorwegen), 18.08.01-21.08.01, p.26 [Downoad abstract] [Download presentation].

Poster presentations:

K. A. Mortier, R. Dams, W. Lambert and A.P. De leenheer. A validated method for the determination of PMA and other amphetamine-related designer drugs in biological matrices by liquid chromatography sonic spray ionization mass spectrometry. Abstracts book of the Seventh International Symposium on Hyphenated Techniques in Chromatography and hyphenated Chromatographic Analyzers (HTC-7), Brugge (Belgium), 06.02.02 - 08.02.02, p112 (Download Abstract Download poster 

R. Dams, T. Benijts, W.E. Lambert and A.P. De Leenheer.  (Fast) simultaneous determination of seven opiates by gradient liquid chromatography/tandem mass spectrometry using a monolithic silica column.    Abstracts book II of the 25th International Symposium on High Performance Liquid Phase Separations and Related Techniques, Maastricht (Nederland), 17.06.01-22.06.01, p.256, (Poster P 1932). Download abstract Download poster

T. Benijts, R. Dams, W. Lambert and A.P. De Leenheer.  Fast analysis of estrogenic compounds by high performance liquid chromatography coupled to ion trap mass spectrometry.  Abstracts book II of the 25th International Symposium on High Performance Liquid Phase Separations and Related Techniques, Maastricht (Nederland), 17.06.01-22.06.01, p.246, (Poster P 1922).[Downoad abstract] 


QTOF system (Micromass)

This high tech piece of equipment is shared with two other laboratories, the Laboratory of Medical Biochemistry and Clinical Analysis and the Laboratory of Pharmaceutical Analysis. The time of flight mass detector is capable to detect in full scan mode with a high sensitivity. This makes it very suitable for proteomics and DNA related research. However, the sensitive full scan mode is also very interesting in toxicology.